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MS fragmentation analysis of the <t>deuterium</t> <t>labeled</t> A1:F3 metabolite, produced in phytol-d6 feeding experiment. (A) Top: Detected ions of A1:F3 corresponding to (1) [M+Na] + , (2) [M-H2O+H]+ and (3) [M-2H2O+H]+. Middle: Fragmentation spectrum (MS 2 ) of (3). (B) Inferred structures of detected ions in the panel A, with broken C-C bonds indicated by the dashed line.
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MS fragmentation analysis of the <t>deuterium</t> <t>labeled</t> A1:F3 metabolite, produced in phytol-d6 feeding experiment. (A) Top: Detected ions of A1:F3 corresponding to (1) [M+Na] + , (2) [M-H2O+H]+ and (3) [M-2H2O+H]+. Middle: Fragmentation spectrum (MS 2 ) of (3). (B) Inferred structures of detected ions in the panel A, with broken C-C bonds indicated by the dashed line.
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MS fragmentation analysis of the <t>deuterium</t> <t>labeled</t> A1:F3 metabolite, produced in phytol-d6 feeding experiment. (A) Top: Detected ions of A1:F3 corresponding to (1) [M+Na] + , (2) [M-H2O+H]+ and (3) [M-2H2O+H]+. Middle: Fragmentation spectrum (MS 2 ) of (3). (B) Inferred structures of detected ions in the panel A, with broken C-C bonds indicated by the dashed line.
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MS fragmentation analysis of the <t>deuterium</t> <t>labeled</t> A1:F3 metabolite, produced in phytol-d6 feeding experiment. (A) Top: Detected ions of A1:F3 corresponding to (1) [M+Na] + , (2) [M-H2O+H]+ and (3) [M-2H2O+H]+. Middle: Fragmentation spectrum (MS 2 ) of (3). (B) Inferred structures of detected ions in the panel A, with broken C-C bonds indicated by the dashed line.
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MS fragmentation analysis of the <t>deuterium</t> <t>labeled</t> A1:F3 metabolite, produced in phytol-d6 feeding experiment. (A) Top: Detected ions of A1:F3 corresponding to (1) [M+Na] + , (2) [M-H2O+H]+ and (3) [M-2H2O+H]+. Middle: Fragmentation spectrum (MS 2 ) of (3). (B) Inferred structures of detected ions in the panel A, with broken C-C bonds indicated by the dashed line.
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MS fragmentation analysis of the deuterium labeled A1:F3 metabolite, produced in phytol-d6 feeding experiment. (A) Top: Detected ions of A1:F3 corresponding to (1) [M+Na] + , (2) [M-H2O+H]+ and (3) [M-2H2O+H]+. Middle: Fragmentation spectrum (MS 2 ) of (3). (B) Inferred structures of detected ions in the panel A, with broken C-C bonds indicated by the dashed line.

Journal: bioRxiv

Article Title: Resolving Phytophthora α2 hormone biosynthesis reveals species-specific mechanisms for mating type regulation

doi: 10.64898/2026.04.23.720443

Figure Lengend Snippet: MS fragmentation analysis of the deuterium labeled A1:F3 metabolite, produced in phytol-d6 feeding experiment. (A) Top: Detected ions of A1:F3 corresponding to (1) [M+Na] + , (2) [M-H2O+H]+ and (3) [M-2H2O+H]+. Middle: Fragmentation spectrum (MS 2 ) of (3). (B) Inferred structures of detected ions in the panel A, with broken C-C bonds indicated by the dashed line.

Article Snippet: To facilitate identification of phytol-derived metabolites, a 100 ml culture of the two strains was prepared containing either unlabeled or deuterium-labeled phytol (Toronto Research Chemicals Inc, Cat. No. TRC-T398919) at 3.4 mM.

Techniques: Labeling, Produced

Journal: bioRxiv

Article Title: Resolving Phytophthora α2 hormone biosynthesis reveals species-specific mechanisms for mating type regulation

doi: 10.64898/2026.04.23.720443

Figure Lengend Snippet:

Article Snippet: To facilitate identification of phytol-derived metabolites, a 100 ml culture of the two strains was prepared containing either unlabeled or deuterium-labeled phytol (Toronto Research Chemicals Inc, Cat. No. TRC-T398919) at 3.4 mM.

Techniques: Labeling